Automated high-throughput high-content autophagy and mitophagy analysis platform.

Jonathan Arias-Fuenzalida, Javier Jarazo, Jonas Walter, Gemma Gomez-Giro, Julia I Forster, Rejko Krueger, Paul M A Antony, Jens C Schwamborn

Scientific reports 2019 Jul 01

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Autophagic processes play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy can be affected at multiple and distinct steps of the pathway. Current analyses tools do not deliver the required detail for dissecting pathway intermediates. The development of new tools to analyze autophagic processes qualitatively and quantitatively in a more straightforward manner is required. Defining all autophagy pathway intermediates in a high-throughput manner is technologically challenging and has not been addressed yet. Here, we overcome those requirements and limitations by the developed of stable autophagy and mitophagy reporter-iPSC and the establishment of a novel high-throughput phenotyping platform utilizing automated high-content image analysis to assess autophagy and mitophagy pathway intermediates.

Citation

Jonathan Arias-Fuenzalida, Javier Jarazo, Jonas Walter, Gemma Gomez-Giro, Julia I Forster, Rejko Krueger, Paul M A Antony, Jens C Schwamborn. Automated high-throughput high-content autophagy and mitophagy analysis platform. Scientific reports. 2019 Jul 01;9(1):9455