Ribosome profiling of selenoproteins in vivo reveals consequences of pathogenic Secisbp2 missense mutations.

Wenchao Zhao, Simon Bohleber, Henrik Schmidt, Sandra Seeher, Michael T Howard, Doreen Braun, Simone Arndt, Uschi Reuter, Hagen Wende, Carmen Birchmeier, Noelia Fradejas-Villar, Ulrich Schweizer

The Journal of biological chemistry 2019 Sep 27

filter terms:

Recoding of UGA codons as selenocysteine (Sec) codons in selenoproteins depends on a selenocysteine insertion sequence (SECIS) in the 3'-UTR of mRNAs of eukaryotic selenoproteins. SECIS-binding protein 2 (SECISBP2) increases the efficiency of this process. Pathogenic mutations in SECISBP2 reduce selenoprotein expression and lead to phenotypes associated with the reduction of deiodinase activities and selenoprotein N expression in humans. Two functions have been ascribed to SECISBP2: binding of SECIS elements in selenoprotein mRNAs and facilitation of co-translational Sec insertion. To separately probe both functions, we established here two mouse models carrying two pathogenic missense mutations in Secisbp2 previously identified in patients. We found that the C696R substitution in the RNA-binding domain abrogates SECIS binding and does not support selenoprotein translation above the level of a complete Secisbp2 null mutation. The R543Q missense substitution located in the selenocysteine insertion domain resulted in residual activity and caused reduced selenoprotein translation, as demonstrated by ribosomal profiling to determine the impact on UGA recoding in individual selenoproteins. We found, however, that the R543Q variant is thermally unstable in vitro and completely degraded in the mouse liver in vivo, while being partially functional in the brain. The moderate impairment of selenoprotein expression in neurons led to astrogliosis and transcriptional induction of genes associated with immune responses. We conclude that differential SECISBP2 protein stability in individual cell types may dictate clinical phenotypes to a much greater extent than molecular interactions involving a mutated amino acid in SECISBP2. © 2019 Zhao et al.

Citation

Wenchao Zhao, Simon Bohleber, Henrik Schmidt, Sandra Seeher, Michael T Howard, Doreen Braun, Simone Arndt, Uschi Reuter, Hagen Wende, Carmen Birchmeier, Noelia Fradejas-Villar, Ulrich Schweizer. Ribosome profiling of selenoproteins in vivo reveals consequences of pathogenic Secisbp2 missense mutations. The Journal of biological chemistry. 2019 Sep 27;294(39):14185-14200