Tatsuya Funai, Megumi Aotani, Risa Kiriu, Junko Nakamura, Yuki Miyazaki, Osamu Nakagawa, Shun-Ichi Wada, Hidetaka Torigoe, Akira Ono, Hidehito Urata
Chembiochem : a European journal of chemical biology 2020 Feb 17Spectroscopic characterization of AgI -ion-mediated C-AgI -A and C-AgI -T base pairs found in primer extension reactions catalyzed by DNA polymerases was conducted. UV melting experiments revealed that C-A and C-T mismatched base pairs in oligodeoxynucleotide duplexes are specifically stabilized by AgI ions in 1:1 stoichiometry in the same manner as a C-C mismatched base pair. Although the stability of the mismatched base pairs in the absence of AgI ions is in the order C-A≈C-T>C-C, the stabilizing effect of AgI ions follows the order C-C>C-A≈C-T. However, the comparative susceptibility of dNTPs to AgI -mediated enzymatic incorporation into the site opposite templating C is dATP>dTTP≫dCTP, as reported. The net charge, as well as the size and/or shape complementarity of the metal-mediated base pairs, or the stabilities of mismatched base pairs in the absence of metal ions, would be more important than the stability of the metallo-base pairs in the replicating reaction catalyzed by DNA polymerases. © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Tatsuya Funai, Megumi Aotani, Risa Kiriu, Junko Nakamura, Yuki Miyazaki, Osamu Nakagawa, Shun-Ichi Wada, Hidetaka Torigoe, Akira Ono, Hidehito Urata. Silver(I)-Ion-Mediated Cytosine-Containing Base Pairs: Metal Ion Specificity for Duplex Stabilization and Susceptibility toward DNA Polymerases. Chembiochem : a European journal of chemical biology. 2020 Feb 17;21(4):517-522
PMID: 31460689
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