Ahmet Engin Pazarçeviren, Tayfun Dikmen, Korhan Altunbaş, Volkan Yaprakçı, Özge Erdemli, Dilek Keskin, Ayşen Tezcaner
Journal of tissue engineering and regenerative medicine 2020 JanIn this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time. Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compression molding technique with varying CLN contents. We hypothesized that CLN reinforcement in a composite scaffold will improve bone regeneration and promote repair. Therefore, the scaffolds were analyzed for compressive strength, biodegradation, biocompatibility, and induction of osteogenic differentiation in vitro. CLN inclusion in PC-10 (10% w/w) and PC-20 (20% w/w) scaffolds revealed 54.7% and 53.4% porosity, higher dry (0.62 and 0.76 MPa), and wet (0.37 and 0.45 MPa) compressive strength, greater cellular adhesion, alkaline phosphatase activity (2.20 and 2.82 mg/gDNA /min), and intracellular calcium concentration (122.44 and 243.24 g Ca/mgDNA ). The scaffolds were evaluated in a unicortical bone defect at anterior aspect of proximal tibia of adult rabbits 4 and 8 weeks postimplantation. Similar to in vitro results, CLN-containing scaffolds led to efficient regeneration of bone in a dose-dependent manner. PC-20 demonstrated highest quality of bone union, cortex development, and bone-scaffold interaction at the defect site. Therefore, higher CLN content in PC-20 permitted robust remodeling whereas pure PCEC (PC-0) scaffolds displayed fibrous tissue formation. Consequently, CLN was proven to be a potent reinforcement in terms of promoting mechanical, physical, and biological properties of polymer-based scaffolds in a more economical, easy-to-handle, and reproducible approach. © 2019 John Wiley & Sons, Ltd.
Ahmet Engin Pazarçeviren, Tayfun Dikmen, Korhan Altunbaş, Volkan Yaprakçı, Özge Erdemli, Dilek Keskin, Ayşen Tezcaner. Composite clinoptilolite/PCL-PEG-PCL scaffolds for bone regeneration: In vitro and in vivo evaluation. Journal of tissue engineering and regenerative medicine. 2020 Jan;14(1):3-15
PMID: 31475790
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