Ultra-low frequency labeling of single cell lineages in Drosophila.

Mark Snee, Wen-Chih Lee, Ryan Beehler-Evans, Craig A Micchelli

Developmental biology 2020 Jan 01

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We describe a new methodology for genetically labeling single cell lineages in Drosophila called DMARCM. The system offers ultra-low frequency labeling, linear induction, consistent labeling among individuals and virtually no background signal. We compare this technique to an existing approach, which has been widely adopted. We demonstrate how application of DMARCM in the gastrointestinal epithelium permits the effects of labeling frequency on tumorigenic stem cell growth to be distinguished in an established tumor model. Copyright © 2019. Published by Elsevier Inc.

Citation

Mark Snee, Wen-Chih Lee, Ryan Beehler-Evans, Craig A Micchelli. Ultra-low frequency labeling of single cell lineages in Drosophila. Developmental biology. 2020 Jan 01;457(1):9-12