Correlation Engine 2.0
Clear Search sequence regions

  • cellular (1)
  • elements (1)
  • factors (4)
  • fibroblasts (1)
  • gene (1)
  • Homeobox Protein (2)
  • mice (1)
  • Nanog Homeobox (2)
  • OCT4 (2)
  • phase (1)
  • Pou5f1 (1)
  • serum (1)
  • stem cells (3)
  • Sizes of these terms reflect their relevance to your search.

    Ectopic transcription factor expression enables reprogramming of somatic cells to pluripotency, albeit with generally low efficiency. Despite steady progress in the field, the exact molecular mechanisms that coordinate this remarkable transition still remain largely elusive. To better characterize the final steps of pluripotency induction, we optimized an experimental system where pluripotent stem cells are differentiated for set intervals before being reintroduced to pluripotency-supporting conditions. Using this approach, we identify a transient period of high-efficiency reprogramming where ectopic transcription factors, but not serum/LIF alone, rapidly revert cells to pluripotency with near 100% efficiency. After this period, cells reprogram with somatic-like kinetics and efficiencies. We identify a set of OCT4 bound cis-regulatory elements that are dynamically regulated during this transient phase and appear central to facilitating reprogramming. Interestingly, these regions remain hypomethylated during in vitro and in vivo differentiation, which may allow them to act as primary targets of ectopically induced factors during somatic cell reprogramming.


    Sudhir Thakurela, Camille Sindhu, Evgeny Yurkovsky, Christina Riemenschneider, Zachary D Smith, Iftach Nachman, Alexander Meissner. Differential regulation of OCT4 targets facilitates reacquisition of pluripotency. Nature communications. 2019 Sep 30;10(1):4444

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 31570708

    View Full Text