A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R.

Priscilla Biswas, Eddi Di Marco, Mauro S Malnati

Methods in molecular biology (Clifton, N.J.) 2020

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The levels of expression of the HLA-class I molecules are critical for modulating T/NK lymphocytes effector functions. Among HLA molecules, HLA-C, the most recent developed form of class I antigens, is subjected to multiple post transcriptional level of regulation that affect its cell surface expression.We describe a new method of allele-specific real-time PCR that monitor the integrity/disruption of the binding site of the microRNA Hsa-miR-148a, a key factor associated to the levels of HLA-C expression in the Caucasian populations.

Citation

Priscilla Biswas, Eddi Di Marco, Mauro S Malnati. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R. Methods in molecular biology (Clifton, N.J.). 2020;2065:55-64