Correlation Engine 2.0
Clear Search sequence regions

  • allele (2)
  • ataxia (1)
  • gait (1)
  • gene (1)
  • hindlimb (1)
  • human (1)
  • impaired (1)
  • mice (3)
  • motor function (1)
  • movement (1)
  • NAV1 6 (2)
  • phenotypes (1)
  • protein domains (1)
  • Scn8a (12)
  • seizures (2)
  • sodium channels (4)
  • suggest (1)
  • tremor (1)
  • Sizes of these terms reflect their relevance to your search.

    Mutations in the voltage-gated sodium channel gene SCN8A cause a broad range of human diseases, including epilepsy, intellectual disability, and ataxia. Here we describe three mouse lines on the C57BL/6J background with novel, overlapping mutations in the Scn8a DIIS4 voltage sensor: an in-frame 9 bp deletion (Δ9), an in-frame 3 bp insertion (∇3) and a 35 bp deletion that results in a frameshift and the generation of a null allele (Δ35). Scn8a Δ9/+ and Scn8a ∇3/+ heterozygous mutants display subtle motor deficits, reduced acoustic startle response, and are resistant to induced seizures, suggesting that these mutations reduce activity of the Scn8a channel protein, Nav 1.6. Heterozygous Scn8a Δ35/+ mutants show no alterations in motor function or acoustic startle response, but are resistant to induced seizures. Homozygous mutants from each line exhibit premature lethality and severe motor impairments, ranging from uncoordinated gait with tremor (Δ9 and ∇3) to loss of hindlimb control (Δ35). Scn8a Δ9/Δ9 and Scn8a ∇3/∇3 homozygous mutants also exhibit impaired nerve conduction velocity, while normal nerve conduction was observed in Scn8a Δ35/Δ35 homozygous mice. Our results suggest that hypomorphic mutations that reduce Nav 1.6 activity will likely result in different clinical phenotypes compared to null alleles. These three mouse lines represent a valuable opportunity to examine the phenotypic impacts of hypomorphic and null Scn8a mutations without the confound of strain-specific differences. © 2019 John Wiley & Sons Ltd and International Behavioural and Neural Genetics Society.


    George Andrew S Inglis, Jennifer C Wong, Kameryn M Butler, Jacquelyn T Thelin, Olivia C Mistretta, Xuewen Wu, Xi Lin, Arthur W English, Andrew Escayg. Mutations in the Scn8a DIIS4 voltage sensor reveal new distinctions among hypomorphic and null Nav 1.6 sodium channels. Genes, brain, and behavior. 2020 Apr;19(4):e12612

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 31605437

    View Full Text