Application of cyclodextrinase in non-complexant production of γ-cyclodextrin.

The production of γ-cyclodextrin usually includes the utilization of organic complexants. However, the non-complexant production of γ-cyclodextrin is always being explored due to the defects of organic complexants. However, in non-complexant production, the separation of γ-cyclodextrin from α- and β-cyclodextrin is still a challenge. Here, the selective hydrolysis ability of a cyclodextrinase designated PpCD (cyclodextrinase from Palaeococcus pacificus) on α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin was proved. The kcat /Km values of PpCD for α-cyclodextrin and β-cyclodextrin were roughly 12-fold and 5-fold higher than that of γ-cyclodextrin. It was proved that PpCD had selective hydrolysis ability and its γ-cyclodextrin purification performance was apparent on various simulated cyclodextrin mixtures with reported proportions derived from different CGTases. Besides, the hydrolysis temperature was optimized and it could be seen that 85°C was appropriate for the production of γ-cyclodextrin. In addition, the production of γ-cyclodextrin was achieved by using PpCD in the γ-CGTase reaction products. © 2019 American Institute of Chemical Engineers.

Citation

Hangyan Ji, Yu Wang, Yuxiang Bai, Xiaoxiao Li, Lizhong Qiu, Zhengyu Jin. Application of cyclodextrinase in non-complexant production of γ-cyclodextrin. Biotechnology progress. 2020 Mar;36(2):e2930

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PMID: 31622540

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