Pre-rRNA processing and its response to temperature stress in maize.

Ribosome biogenesis is a fundamental process in all eukaryotic cells and is coupled with the processing and maturation of pre-rRNAs. Maize is a primary staple crop across the world, but little is known about the exact pre-rRNA processing sites and pathways in this species. In this study, we present a detailed model of the pathway by identifying the critical endonucleolytic cleavage sites and determining the pre-rRNA intermediates by circular reverse-transcription PCR and northern blot analysis. We demonstrate that two pathways coexist in maize to promote the processing of 35S pre-rRNA, and that the processing of 27SA pre-rRNA can proceed via two different pathways, which are distinguished based on the order of ITS1 removal and ITS2 cleavage. Compared with yeast and mammals, this new 27SA pre-rRNA processing mechanism is unique to maize and other higher plants. In addition, we demonstrate that maize can modulate pre-rRNA processing levels in response to chilling and heat stress, as indicated by a significant reduction of the P-A3 intermediate. Our study provides information that will facilitate future research on ribosome biogenesis and pre-rRNA processing in maize. © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Citation

Guoqin Liu, Pengshuai Yan, Qingguo Du, Yafei Wang, Yu Guo, Zhiyuan Fu, Hongqiu Wang, Jihua Tang. Pre-rRNA processing and its response to temperature stress in maize. Journal of experimental botany. 2020 Feb 19;71(4):1363-1374

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PMID: 31665749

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