Correlation Engine 2.0
Clear Search sequence regions


  • adult (1)
  • HDL2 (1)
  • HDL3 (1)
  • help (1)
  • humans (1)
  • lipoprotein (12)
  • lipoproteins hdl (2)
  • lipoproteins hdl2 (2)
  • lipoproteins hdl3 (3)
  • mass (1)
  • parallel (4)
  • peptides (1)
  • proteomes (2)
  • young adult (1)
  • Sizes of these terms reflect their relevance to your search.

    High-density lipoprotein (HDL) is a diverse group of particles with multiple cardioprotective functions. HDL proteome follows HDL particle complexity. Many proteins were described in HDL, but consistent quantification of HDL protein cargo is still a challenge. To address this issue, the aim of this work was to compare data-independent acquisition (DIA) and parallel reaction monitoring (PRM) methodologies in their abilities to differentiate HDL subclasses through their proteomes. To this end, we first evaluated the analytical performances of DIA and PRM using labeled peptides in pooled digested HDL as a biological matrix. Next, we compared the quantification capabilities of the two methodologies for 24 proteins found in HDL2 and HDL3 from 19 apparently healthy subjects. DIA and PRM exhibited comparable linearity, accuracy, and precision. Moreover, both methodologies worked equally well, differentiating HDL subclasses' proteomes with high precision. Our findings may help to understand HDL functional diversity.

    Citation

    Amanda R M Silva, Marcos T K Toyoshima, Marisa Passarelli, Paolo Di Mascio, Graziella E Ronsein. Comparing Data-Independent Acquisition and Parallel Reaction Monitoring in Their Abilities To Differentiate High-Density Lipoprotein Subclasses. Journal of proteome research. 2020 Jan 03;19(1):248-259

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 31697504

    View Full Text