Labeling Acidic Compartments of Neutrophils with Cresyl Violet.

Philip P Ostrowski, Ziv Roth, Sergio Grinstein

Methods in molecular biology (Clifton, N.J.) 2020

filter terms:

We introduce the acidotropic marker cresyl violet to stain acidic granules in live neutrophils. Cresyl violet is less phototoxic, more photostable, and more cost-effective than other commercially available acidotropic markers. Additionally, it does not photoconvert to fluorescent species of a different color, a limitation of other commonly used acidotropic markers. Staining can be readily detected by fluorescence microscopy or by flow cytometry, and can be used as a readout of degranulation in activated neutrophils.

Citation

Philip P Ostrowski, Ziv Roth, Sergio Grinstein. Labeling Acidic Compartments of Neutrophils with Cresyl Violet. Methods in molecular biology (Clifton, N.J.). 2020;2087:207-213