Inhibition of Ctsk modulates periodontitis with arthritis via downregulation of TLR9 and autophagy.

Wei Wei, Jie Ren, Wuwei Yin, Handong Ding, Qiuyu Lu, Liangyu Tan, Shibing Deng, Jie Liu, Qin Yang, Jiajia Wang, Min Wang, Yuan Yue, Liang Hao

Cell proliferation 2020 Jan

filter terms:

The mechanisms underlying the effects of Toll-like receptor 9 (TLR9) and autophagy on rheumatoid arthritis (RA)-aggravated periodontitis are unclear. We aimed to explore a novel target, cathepsin K (Ctsk)-mediated TLR9-related autophagy, during the progress of periodontitis with RA. DBA/J1 mouse model of periodontitis with RA was created by local colonization of Porphyromonas gingivalis (Pg) and injection of collagen. The expression of Ctsk was inhibited by adeno-associated virus (AAV). Micro-CT, immunohistochemistry (IHC), Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were used to detect the expression of TLR9-related autophagy in periodontitis with RA. Small interfering RNA (siRNA) and CpG oligodeoxynucleotides (CpG ODN) were applied in macrophages. Western blot, immunofluorescence (IF) and qRT-PCR were used to verify the in vivo results. RA can promote periodontitis bone destruction in the lesion area, while inhibiting Ctsk could effectively alleviate this effect. The infiltration of macrophages, TLR9, autophagy proteins (TFEB and LC3) and inflammatory cytokines increased in the periodontitis-with-RA group and was reduced by the inhibition of Ctsk in the periodontal region. Macrophage stimulation confirmed the in vivo results. With the activation of TLR9 by CpG ODN, inhibition of Ctsk could suppress both TLR9 downstream signalling proteins and autophagy-related proteins. This study advanced a novel role for Ctsk in TLR9 and autophagy to explain the interaction between periodontitis and RA. © 2019 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.

Citation

Wei Wei, Jie Ren, Wuwei Yin, Handong Ding, Qiuyu Lu, Liangyu Tan, Shibing Deng, Jie Liu, Qin Yang, Jiajia Wang, Min Wang, Yuan Yue, Liang Hao. Inhibition of Ctsk modulates periodontitis with arthritis via downregulation of TLR9 and autophagy. Cell proliferation. 2020 Jan;53(1):e12722