Circ-HIPK3 plays an active role in regulating myoblast differentiation.

Rui Yao, Yang Yao, Cunyuan Li, Xiaoyue Li, Wei Ni, Renzhe Quan, Li Liu, Huixiang Li, Yueren Xu, Mengdan Zhang, Yaseen Ullah, Shengwei Hu

International journal of biological macromolecules 2020 Jul 15

filter terms:

The development of skeletal muscle is a complex biological process involving a variety of regulatory pathways. A deeper understanding of the developmental mechanisms of skeletal muscle is of great significance for the treatment of muscular lesions. In recent years, studies have shown that circRNAs, as a new class of post-transcriptional regulators, play an important regulatory role in a variety of biological processes, but their function in skeletal muscle development remains unclear. Here, we used C2C12 myoblasts to study circ-HIPK3, which has significant differences in the developmental stages of skeletal muscle and is highly expressed. We found that the expression level of circ-HIPK3 was significantly up-regulated (p < .05) with the persistence of C2C12 cell differentiation. Combining the results of bioinformatics analysis and dual luciferase reporter experiments, we found that circ-HIPK3 is a sponge of miR-124 and miR-379 that regulate muscle differentiation. Our study shows that circ-HIPK3 can promote the differentiation of C2C12 myoblasts, providing a scientific basis for further research on the development of skeletal muscle at the level of circRNAs. Copyright © 2019. Published by Elsevier B.V.

Citation

Rui Yao, Yang Yao, Cunyuan Li, Xiaoyue Li, Wei Ni, Renzhe Quan, Li Liu, Huixiang Li, Yueren Xu, Mengdan Zhang, Yaseen Ullah, Shengwei Hu. Circ-HIPK3 plays an active role in regulating myoblast differentiation. International journal of biological macromolecules. 2020 Jul 15;155:1432-1439