The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis.

Qudsia Zeb, Xiaohan Wang, Congcong Hou, Xiwen Zhang, Mengqi Dong, Sisi Zhang, Qian Zhang, Zhijie Ren, Wang Tian, Huifen Zhu, Legong Li, Liangyu Liu

Journal of integrative plant biology 2020 Jul

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Oscillations in cytosolic free calcium determine the polarity of tip-growing root hairs. The Ca2+ channel cyclic nucleotide gated channel 14 (CNGC14) contributes to the dynamic changes in Ca2+ concentration gradient at the root hair tip. However, the mechanisms that regulate CNGC14 are unknown. In this study, we detected a direct interaction between calmodulin 7 (CaM7) and CNGC14 through yeast two-hybrid and bimolecular fluorescence complementation assays. We demonstrated that the third EF-hand domain of CaM7 specifically interacts with the cytosolic C-terminal domain of CNGC14. A two-electrode voltage clamp assay showed that CaM7 completely inhibits CNGC14-mediated Ca2+ influx, suggesting that CaM7 negatively regulates CNGC14-mediated calcium signaling. Furthermore, CaM7 overexpressing lines phenocopy the short root hair phenotype of a cngc14 mutant and this phenotype is insensitive to changes in external Ca2+ concentrations. We, thus, identified CaM7-CNGC14 as a novel interacting module that regulates polar growth in root hairs by controlling the tip-focused Ca2+ signal. © 2019 Institute of Botany, Chinese Academy of Sciences.

Citation

Qudsia Zeb, Xiaohan Wang, Congcong Hou, Xiwen Zhang, Mengqi Dong, Sisi Zhang, Qian Zhang, Zhijie Ren, Wang Tian, Huifen Zhu, Legong Li, Liangyu Liu. The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis. Journal of integrative plant biology. 2020 Jul;62(7):887-896