Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate.

Akihiko Tanimura, Satoshi Shuto

Methods in molecular biology (Clifton, N.J.) 2020

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We present a novel method, termed competitive fluorescent ligand assay for inositol 1,4,5-trisphosphate (CFLA-IP3), to measure inositol 1,4,5-trisphosphate (IP3). This method is based on fluorescence resonance energy transfer (FRET) between two fluorescent molecules, a fluorescent IP3-binding protein and its fluorescent ligand. Binding of these fluorescent molecules generates a FRET signal, and the IP3-dependent decrease in the FRET signal due to displacement of the fluorescent ligand is detected by fluorescence microscopy.

Citation

Akihiko Tanimura, Satoshi Shuto. Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate. Methods in molecular biology (Clifton, N.J.). 2020;2091:137-144