Structure analysis of polysaccharides purified from Cyclocarya paliurus with DEAE-Cellulose and its antioxidant activity in RAW264.7 cells.

Qi An, Ximei Ye, Yi Han, Meng Zhao, Si Chen, Xin Liu, Xiang Li, Zitong Zhao, Yang Zhang, Kehui Ouyang, Wenjun Wang

International journal of biological macromolecules 2020 Aug 15

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CPP was isolated from Cyclocarya paliurus (C. paliurus) and CPP-D was purified from CPP with a further step by DEAE-Cellulose. In this study, the structure and antioxidant activities of these two polysaccharides were investigated. The molecular weight of CPP was determined as 1.15 × 105 Da and the monosaccharides of it were Rha, Ara, Xyl, Man, Glc, Gal in a molar ratio of 0.021:0.237:0.020:0.036:0.454:0.231, while the molecular weight of CPP-D was 9.1 × 103 Da and the monosaccharides of it were Man, Glc, Gal in a molar ratio of 0.235:0.677:0.088. CPP-D consisted of three structural residues →4)-β-D-Glc-(1→, →2,6)-β-D-Man-(1→ and →4)-β-D-Gal. These structures were characterized by SEM, FT-IR, GC-MS, HPGPC, and NMR. The antioxidant assay in RAW264.7 cell showed that both CPP and CPP-D promoted cell viability and antioxidant activity, which decreased the content of MDA and increased the activity of SOD, T-AOC, CAT (P < .05). As a result, CPP-D isolated by DEAE-Cellulose didn't reduce the antioxidant activity of C. paliurus polysaccharide and could enhance the cell viability. Copyright © 2019 Elsevier B.V. All rights reserved.

Citation

Qi An, Ximei Ye, Yi Han, Meng Zhao, Si Chen, Xin Liu, Xiang Li, Zitong Zhao, Yang Zhang, Kehui Ouyang, Wenjun Wang. Structure analysis of polysaccharides purified from Cyclocarya paliurus with DEAE-Cellulose and its antioxidant activity in RAW264.7 cells. International journal of biological macromolecules. 2020 Aug 15;157:604-615