The analysis of organization and diversity mechanism in goat immunoglobulin light chain gene loci.

Xiaohui Yu, Lijuan Du, Mingli Wu, Jie Wu, Shuai He, Tingting Yuan, Xiuzhu Sun

Immunobiology 2020 Mar

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The genomic organization of goat immunoglobulin light chains (Igλ and Igκ) loci were annotated based on the goat genome database. The goat Igλ chain located on chromosome 17 contains at least 35 Vλ gene fragments (seven potential functional genes, one ORF and 27 pseudogenes), two Jλ-Cλ clusters arranged in a Vλ(35)-Jλ2-Cλ1-Jλ1-Cλ2 pattern, with another Cλ3 on scaffold. The Igκ locus included 11 Vκ (five potential functional genes, two ORFs and four pseudogene fragments), three Jκ genes and a single Cκ gene ordered in Vκ(35)-Jκ(3)-Cκ pattern on chromosome 11. By analyzing the clonies of Igλ and Igκ, we further found Vλ2 (26.23 %) &Vλ3 (73.11 %), Vκ2 (52.07 %) &Vκ4 (46.15 %) were predominately used in the expression of λ and κ chains respectively. λ chain showed more abundance in connective diversity than κ chain. Besides, somatic hypermutation with higher frequency in both immunoglobulin light chains was the major mechanism for the goat repertoire diversity. These results demonstrated goat immunoglobulin light chain variable region genome loci and repertoire diversity. Copyright © 2019 Elsevier GmbH. All rights reserved.

Citation

Xiaohui Yu, Lijuan Du, Mingli Wu, Jie Wu, Shuai He, Tingting Yuan, Xiuzhu Sun. The analysis of organization and diversity mechanism in goat immunoglobulin light chain gene loci. Immunobiology. 2020 Mar;225(2):151889