Rewiring Ancient Residue Interaction Networks Drove the Evolution of Specificity in Steroid Receptors.

C Denise Okafor, David Hercules, Steven A Kell, Eric A Ortlund

Structure (London, England : 1993) 2020 Feb 04

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Understanding how changes in amino acid sequence alter protein dynamics and allosteric signaling would illuminate strategies for protein design. To gain insight into this process, we have combined molecular dynamics simulations with ancestral sequence reconstruction to explore conformational dynamics in two ancient steroid receptors (SRs) to determine how allosteric signaling pathways were altered over evolution to generate hormone specificity. In a broad panel of aromatized and non-aromatized hormones, we investigate inter-residue contacts that facilitate allosteric signaling. This work reveals interhelical interactions that act as ligand sensors and explain the structural and dynamical basis for ligand discrimination in SRs. These sensors are part of a conserved SR allosteric network and persist over long simulation time scales, indicating that evolutionary substitutions rewire ancient SR networks to achieve functional evolution. This powerful combination of computation, ancestral reconstruction, and biochemistry may illuminate allosteric mechanisms and functional evolution in other protein families. Copyright © 2019 Elsevier Ltd. All rights reserved.

Citation

C Denise Okafor, David Hercules, Steven A Kell, Eric A Ortlund. Rewiring Ancient Residue Interaction Networks Drove the Evolution of Specificity in Steroid Receptors. Structure (London, England : 1993). 2020 Feb 04;28(2):196-205.e3