Genome of Bacillus sp. strain QHF158 provides insights into its parasporal inclusions encoded by the S-layer gene.

Bacillus sp. strain QHF158, a Gram-positive, spore-forming and parasporal crystal-secreting bacterium, was isolated from soil of Limushan National Forest Park in China. Here we present the significant feature of parasporal inclusions of this organism, together with the draft genome sequence and annotation. Phylogenetic analysis suggested that strain QHF158 is possibly a novel species, most closely related to Bacillus mycoides. Genome annotation results revealed that strain QHF158 did not contain any typical Cry or Cyt toxin coding gene. Furthermore, the mass spectrometry analyses demonstrated that the parasporal crystalline inclusions were encoded by the orf_05273 gene, with 95% similarity to the S-layer protein (SLP) EA1 of B. mycoides, which indicated that the parasporal crystal from Bacillus sp. strain QHF158 was mainly formed by SLP, instead of the typical Cry or Cyt toxin proteins.

Citation

Yinghong Jin, Junhui Wang, Qian Liu, Haifang Qiu, Yangyang Wang, Jiayi Zhou, Jie Sun, Peng Li, Ruiping Wang, Wenfei Zhang. Genome of Bacillus sp. strain QHF158 provides insights into its parasporal inclusions encoded by the S-layer gene. Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. 2020 Sep;51(3):1145-1150

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PMID: 31836963

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