Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

Salmonella is a human and animal pathogen that causes gastro-enteric diseases. The key to Salmonella infection is its entry into intestinal epithelial cells, where the bacterium resides within a Salmonella-containing vacuole (SCV). Salmonella entry also induces the formation of empty macropinosomes, distinct from the SCV, in the vicinity of the entering bacteria. A few minutes after its formation, the SCV increases in size through fusions with the surrounding macropinosomes. Salmonella also induces membrane tubules that emanate from the SCV and lead to SCV shrinkage. Here, we show that these antipodal events are utilized by Salmonella to either establish a vacuolar niche or to be released into the cytosol by SCV rupture. We identify the molecular machinery underlying dynamic SCV growth and shrinkage. In particular, the SNARE proteins SNAP25 and STX4 participate in SCV inflation by fusion with macropinosomes. Thus, host compartment size control emerges as a pathogen strategy for intracellular niche regulation. Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Virginie Stévenin, Yuen-Yan Chang, Yoann Le Toquin, Magalie Duchateau, Quentin Giai Gianetto, Chak Hon Luk, Audrey Salles, Victoria Sohst, Mariette Matondo, Norbert Reiling, Jost Enninga. Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche. Cell reports. 2019 Dec 17;29(12):3958-3973.e7

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 31851926

View Full Text