Stéphan Hardivillé, Partha S Banerjee, Ebru S Selen Alpergin, Danielle M Smith, Guanghui Han, Junfeng Ma, C Conover Talbot, Ping Hu, Michael J Wolfgang, Gerald W Hart
Molecular cell 2020 Mar 05In eukaryotes, gene expression is performed by three RNA polymerases that are targeted to promoters by molecular complexes. A unique common factor, the TATA-box binding protein (TBP), is thought to serve as a platform to assemble pre-initiation complexes competent for transcription. Here, we describe a novel molecular mechanism of nutrient regulation of gene transcription by dynamic O-GlcNAcylation of TBP. We show that O-GlcNAcylation at T114 of TBP blocks its interaction with BTAF1, hence the formation of the B-TFIID complex, and its dynamic cycling on and off of DNA. Transcriptomic and metabolomic analyses of TBPT114A CRISPR/Cas9-edited cells showed that loss of O-GlcNAcylation at T114 increases TBP binding to BTAF1 and directly impacts expression of 408 genes. Lack of O-GlcNAcylation at T114 is associated with a striking reprogramming of cellular metabolism induced by a profound modification of the transcriptome, leading to gross alterations in lipid storage. Copyright © 2019 Elsevier Inc. All rights reserved.
Stéphan Hardivillé, Partha S Banerjee, Ebru S Selen Alpergin, Danielle M Smith, Guanghui Han, Junfeng Ma, C Conover Talbot, Ping Hu, Michael J Wolfgang, Gerald W Hart. TATA-Box Binding Protein O-GlcNAcylation at T114 Regulates Formation of the B-TFIID Complex and Is Critical for Metabolic Gene Regulation. Molecular cell. 2020 Mar 05;77(5):1143-1152.e7
PMID: 31866147
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