Comparison of plasma membrane H+-ATPase response to acid rain stress between rice and soybean.

Chanjuan Liang, Yongjia Ma, Lingrui Li

Environmental science and pollution research international 2020 Feb

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Acid rain is a global environmental issue due to inhibiting severely plant growth and productivity. To discover the tolerant mechanism in plants under acid rain stress, we studied the difference in response of two crops (rice and soybean) to simulated acid rain (pH 5.0 ~ 2.5) at growth and physiological, biochemical and molecular levels during exposure and recovery periods by hydroponics. By analyzing the change in relative growth rate, chlorophyll content and plasma permeability in rice and soybean, we found that rice could tolerate acid rain above pH 3.0 whereas soybean could tolerate acid rain above pH 4.5. By RT-PCR analyses, immunoprecipitation and enzyme kinetics study, we observed that pH 4.5 acid rain promoted the transcriptional expression of H+-ATPase genes and the phosphorylation of H+-ATPase and increased H+-ATPase activity in the two crops for resisting acid stress. The increased degree in soybean was larger than that in rice. Acid rain at pH 3.0 still promoted the transcription regulation to maintain H+-ATPase activity higher in rice for resisting stress but caused irreversible inhibition on express of H+-ATPase and decreased H+-ATPase activity in soybean. All results suggest that the different tolerance in rice and soybean to acid rain stress could be associated with difference in plasma membrane H+-ATPase at transcriptional regulation, post-translational modification and the substrate affinity.

Citation

Chanjuan Liang, Yongjia Ma, Lingrui Li. Comparison of plasma membrane H+-ATPase response to acid rain stress between rice and soybean. Environmental science and pollution research international. 2020 Feb;27(6):6389-6400