Rhizosphere modelling reveals spatiotemporal distribution of daidzein shaping soybean rhizosphere bacterial community.

Fuki Okutani, Shoichiro Hamamoto, Yuichi Aoki, Masaru Nakayasu, Naoto Nihei, Taku Nishimura, Kazufumi Yazaki, Akifumi Sugiyama

Plant, cell & environment 2020 Apr

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Plant roots nurture a wide variety of microbes via exudation of metabolites, shaping the rhizosphere's microbial community. Despite the importance of plant specialized metabolites in the assemblage and function of microbial communities in the rhizosphere, little is known of how far the effects of these metabolites extend through the soil. We employed a fluid model to simulate the spatiotemporal distribution of daidzein, an isoflavone secreted from soybean roots, and validated using soybeans grown in a rhizobox. We then analysed how daidzein affects bacterial communities using soils artificially treated with daidzein. Simulation of daidzein distribution showed that it was only present within a few millimetres of root surfaces. After 14 days in a rhizobox, daidzein was only present within 2 mm of root surfaces. Soils with different concentrations of daidzein showed different community composition, with reduced α-diversity in daidzein-treated soils. Bacterial communities of daidzein-treated soils were closer to those of the soybean rhizosphere than those of bulk soils. This study highlighted the limited distribution of daidzein within a few millimetres of root surfaces and demonstrated a novel role of daidzein in assembling bacterial communities in the rhizosphere by acting as more of a repellant than an attractant. © 2019 John Wiley & Sons Ltd.

Citation

Fuki Okutani, Shoichiro Hamamoto, Yuichi Aoki, Masaru Nakayasu, Naoto Nihei, Taku Nishimura, Kazufumi Yazaki, Akifumi Sugiyama. Rhizosphere modelling reveals spatiotemporal distribution of daidzein shaping soybean rhizosphere bacterial community. Plant, cell & environment. 2020 Apr;43(4):1036-1046