Long non-coding RNA HULC exerts oncogenic activity on papillary thyroid cancer in vitro and in vivo.

Zhijia Yang, Guoqing Li, Chao Ding, Wencong Sun, Ji Zhang

Artificial cells, nanomedicine, and biotechnology 2020 Dec

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Thyroid cancer is a frequently happened malignancy in human endocrine system. Papillary thyroid cancer (PTC) presents 70-80% of all thyroid cancer cases. Herein, we probed the possible oncogenic function of long non-coding RNA (lncRNA) highly up-regulated in liver cancer (HULC) in PTC. First, the HULC and microRNA-106a (miR-106a) expressions in PTC tissues and cells were tested. Plasmids or miRNAs transfections were done for altering HULC and miR-106a expressions. Then, cells viability and apoptosis, along with cell proliferative, migratory and invasive abilities, were tested, respectively. The PI3K/AKT and Wnt/β-catenin pathways activities were measured. Finally, the animal model of PTC was constructed and the tumour volumes and weights were gauged. We discovered that HULC and miR-106a had relative high expression levels in PTC tissues and cells. HULC overexpression enhanced TPC-1 cells viability and cell proliferative, migratory and invasive abilities. Silencing HULC induced TPC-1 cell apoptosis. miR-106a engaged in the oncogenic impacts of HULC. Moreover, HULC overexpression boosted PI3K/AKT and Wnt/β-catenin pathways activities via raising miR-106a expression. Besides, HULC overexpression enhanced the volumes and weights of PTC tumours. To sum up, HULC exhibited oncogenic function on PTC in vitro and in vivo.

Citation

Zhijia Yang, Guoqing Li, Chao Ding, Wencong Sun, Ji Zhang. Long non-coding RNA HULC exerts oncogenic activity on papillary thyroid cancer in vitro and in vivo. Artificial cells, nanomedicine, and biotechnology. 2020 Dec;48(1):326-335