A novel simple genotyping assay for detection of the 'Gait keeper' mutation in DMRT3 and allele frequencies in Azteca and Costa Rican Saddle Horse breeds.

Miguel Angel Ayala-Valdovinos, Jorge Galindo-García, David Sánchez-Chiprés, Theodor Duifhuis-Rivera, Rubén Anguiano-Estrella

Molecular and cellular probes 2020 Apr

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The 'Gait keeper' mutation in the DMRT3 gene alters locomotion and gait patterns in horses. This mutation (C>A) has been found in all gaited breeds of horses analyzed but is absent in most non-gaited breeds. We developed a new mutagenically separated polymerase chain reaction (MS-PCR) based method for simple detection of horse DMRT3 genotype. Our method was applied in a preliminary study to determine DMRT3 allele frequencies in 78 Azteca horses (AZ) and 53 Costa Rican Saddle Horses (CRSH). We found a wild-type C allele frequency of 100% in the AZ horses. For the CRSH, the wild-type C frequency and mutant A allele frequency were 88.7% and 11.3%, respectively. Copyright © 2020 Elsevier Ltd. All rights reserved.

Citation

Miguel Angel Ayala-Valdovinos, Jorge Galindo-García, David Sánchez-Chiprés, Theodor Duifhuis-Rivera, Rubén Anguiano-Estrella. A novel simple genotyping assay for detection of the 'Gait keeper' mutation in DMRT3 and allele frequencies in Azteca and Costa Rican Saddle Horse breeds. Molecular and cellular probes. 2020 Apr;50:101506