Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita.

Iliyas Rashid, Vishwamitra Singh Baisvar, Mahender Singh, Ravindra Kumar, Prachi Srivastava, Basdeo Kushwaha, Ajey Kumar Pathak

Genomics 2020 May

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The HMOX1 gene plays role in several biological processes and is also responsive to hypoxia stress. Freshwater carp fish, Labeo rohita, is reported as hypoxia sensitive, but the information of annotated hypoxia genes in public domain is very scanty for this species. Here, an attempt was made to isolate and characterize HMOX1 gene in L. rohita using information from zebrafish. HMOX1 gene was obtained by mapping HMOX1 protein of zebrafish over assembled genome of L. rohita. Aligned region was used for designing primers for HMOX1 amplification. Eight overlapping sets of primers were designed for amplifying ~540 bp long successive overlapping fragments. Splicing of overlapping amplicons generated 3715 bp fragment that was confirmed as HMOX1 gene having full coding region with 6 exons between 184 and 2156 bp positions. HMOX1 characterization is an initiative for L. rohita genes annotation to support the characterization of new genes in the important species. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Iliyas Rashid, Vishwamitra Singh Baisvar, Mahender Singh, Ravindra Kumar, Prachi Srivastava, Basdeo Kushwaha, Ajey Kumar Pathak. Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita. Genomics. 2020 May;112(3):2327-2333