Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production.

Matthew N Henry, Michael A MacDonald, Camila A Orellana, Peter P Gray, Marianne Gillard, Kym Baker, Lars K Nielsen, Esteban Marcellin, Stephen Mahler, Verónica S Martínez

Biotechnology and bioengineering 2020 Apr

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Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells. © 2020 Wiley Periodicals, Inc.

Citation

Matthew N Henry, Michael A MacDonald, Camila A Orellana, Peter P Gray, Marianne Gillard, Kym Baker, Lars K Nielsen, Esteban Marcellin, Stephen Mahler, Verónica S Martínez. Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production. Biotechnology and bioengineering. 2020 Apr;117(4):1187-1203