Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.

Herui Wang, Ryan L Pangilinan, Yan Zhu

Methods in molecular biology (Clifton, N.J.) 2020

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Tyramide signal amplification (TSA) is an enzyme-mediated method to enhance the immunohistochemical detection of protein, nucleic acid, or other molecules in situ.Here we describe immunofluorescent detection of a low-abundance cytokine receptor, interleukin-17 receptor B (IL17RB) in U2OS cells, using tyramide signal amplification. In addition, we present a tyramide signal amplification compatible double-color immunostaining protocol using primary antibodies from the same host species. Those applications allow detection of cellular proteins with enhanced sensitivity and add flexibility on primary antibody selection in double- or multicolor immunofluorescence staining assays.

Citation

Herui Wang, Ryan L Pangilinan, Yan Zhu. Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence. Methods in molecular biology (Clifton, N.J.). 2020;2108:89-97