Correlation Engine 2.0
Clear Search sequence regions

  • antibiotics (3)
  • e coli (8)
  • ereA (8)
  • erythromycin (8)
  • escherichia coli (3)
  • ester (2)
  • feces (3)
  • female (1)
  • genes (1)
  • hydrolases (2)
  • livestock (2)
  • mice (5)
  • pcr (1)
  • Sizes of these terms reflect their relevance to your search.

    Antibiotics are widely used in livestock and poultry industries, which results in large quantities of antibiotic residues in manure that influences subsequent treatments. In this study, an Escherichia coli strain was engineered to display erythromycin esterase on its cell surface. The engineered strain (E. coli ereA) efficiently degraded erythromycin by opening the macrocyclic 14-membered lactone ring in solution. Erythromycin (50 mg/L) was completely degraded in a solution by E. coli ereA (1 × 109 CFU/mL) within 24 h. E. coli ereA retained over 86.7 % of the initial enzyme activity after 40 days of storage at 25 °C, and 78.5 % of the initial activity after seven repeated batch reactions in solution at 25 °C. Mice were fed with E. coli ereA and real-time quantitative PCR data showed that E. coli ereA colonized in the mice large intestine. The mice group fed E. coli ereA exhibited 83.13 % decrease in erythromycin levels in their feces compared with the mice group not fed E. coli ereA. E. coli ereA eliminated antibiotics from the source preventing its release into the environment. The surface-engineered strain therefore is an effective alternative agent for treating recalcitrant antibiotics, and has the potential to be applied in livestock and poultry industries. Copyright © 2020 Elsevier B.V. All rights reserved.


    Minrui Liu, Pengya Feng, Apurva Kakade, Ling Yang, Gang Chen, Xiaojun Yan, Hongyuhang Ni, Pu Liu, Saurabh Kulshreshtha, Abd El-Fatah Abomohra, Xiangkai Li. Reducing residual antibiotic levels in animal feces using intestinal Escherichia coli with surface-displayed erythromycin esterase. Journal of hazardous materials. 2020 Apr 15;388:122032

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 31955024

    View Full Text