Involvement of GPR4 in increased growth hormone and prolactin expressions by extracellular acidification in MtT/S cells.

Shiori Musha, Saishu Yoshida, Syo Murakami, Ryotaro Kojima, Masahito Deai, Naoshi Saso, Chihiro Mogi, Koichi Sato, Fumikazu Okajima, Hideaki Tomura

The Journal of reproduction and development 2020 Apr 10

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Hormone-secreting pituitary adenomas show unregulated hormonal hypersecretion and cause hyperpituitarism. However, the mechanism of the unregulated hormone production and secretion has not yet been fully elucidated. Solid tumors show reduced extracellular pH, partly due to lactate secretion from anaerobic glycolysis. It is known that extracellular acidification affects hormone secretion. However, whether and how the extracellular acidification influences the unregulated hormone production and secretion remain unknown. In the present study, we found that GPR4, a proton-sensing G protein-coupled receptor, was highly expressed in MtT/S cells, a growth hormone-producing and prolactin-producing pituitary tumor cell line. When we reduced the extracellular pH, growth hormone and prolactin mRNA expressions increased in the cells. Both increased expressions were partially suppressed by a GPR4 antagonist. We also found that extracellular acidification enhanced growth hormone-releasing factor-induced growth hormone secretion from MtT/S cells. These results suggest that GPR4 may play a role in hypersecretion of the hormone from hormone-producing pituitary tumors. A GPR4 antagonist will be a useful tool for preventing the hypersecretion.

Citation

Shiori Musha, Saishu Yoshida, Syo Murakami, Ryotaro Kojima, Masahito Deai, Naoshi Saso, Chihiro Mogi, Koichi Sato, Fumikazu Okajima, Hideaki Tomura. Involvement of GPR4 in increased growth hormone and prolactin expressions by extracellular acidification in MtT/S cells. The Journal of reproduction and development. 2020 Apr 10;66(2):175-180