Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins.

Bernd Gardill, Jerry Huang, Lawrence Tu, Filip Van Petegem, Kirill Oxenoid, Christy A Thomson

Scientific reports 2020 Jan 24

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Multi-pass membrane proteins are important targets of biologic medicines. Given the inherent difficulties in working with membrane proteins, we sought to investigate the utility of membrane scaffold protein nanodiscs as a means of solubilizing membrane proteins to aid antibody discovery. Using a model multi-pass membrane protein, we demonstrate how incorporation of a multi-pass membrane protein into nanodiscs can be used in flow cytometry to identify antigen-specific hybridoma. The use of target protein-loaded nanodiscs to sort individual hybridoma early in the screening process can reduce the time required to identify antibodies against multi-pass membrane proteins.

Citation

Bernd Gardill, Jerry Huang, Lawrence Tu, Filip Van Petegem, Kirill Oxenoid, Christy A Thomson. Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins. Scientific reports. 2020 Jan 24;10(1):1130