Structural basis for electron transport mechanism of complex I-like photosynthetic NAD(P)H dehydrogenase.

NAD(P)H dehydrogenase-like (NDH) complex NDH-1L of cyanobacteria plays a crucial role in cyclic electron flow (CEF) around photosystem I and respiration processes. NDH-1L couples the electron transport from ferredoxin (Fd) to plastoquinone (PQ) and proton pumping from cytoplasm to the lumen that drives the ATP production. NDH-1L-dependent CEF increases the ATP/NADPH ratio, and is therefore pivotal for oxygenic phototrophs to function under stress. Here we report two structures of NDH-1L from Thermosynechococcus elongatus BP-1, in complex with one Fd and an endogenous PQ, respectively. Our structures represent the complete model of cyanobacterial NDH-1L, revealing the binding manner of NDH-1L with Fd and PQ, as well as the structural elements crucial for proper functioning of the NDH-1L complex. Together, our data provides deep insights into the electron transport from Fd to PQ, and its coupling with proton translocation in NDH-1L.

Citation

Xiaowei Pan, Duanfang Cao, Fen Xie, Fang Xu, Xiaodong Su, Hualing Mi, Xinzheng Zhang, Mei Li. Structural basis for electron transport mechanism of complex I-like photosynthetic NAD(P)H dehydrogenase. Nature communications. 2020 Jan 30;11(1):610

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PMID: 32001694

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