Analysis of the expression of transglutaminases in the reconstructed human epidermis using a three-dimensional cell culture.

Hirofumi Teshima, Manami Kato, Hideki Tatsukawa, Kiyotaka Hitomi

Analytical biochemistry 2020 Aug 15

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The skin epidermis functions as a barrier to various external stresses. In the outermost layer, the terminally differentiated keratinocytes result in cornification with a tough structure by formation of a cornified envelope beneath the plasma membrane. To complete the formation of the cornified envelope, several structural proteins are cross-linked via the catalytic action of transglutaminases (TG1, TG3, TG5, and TG6). The expression and activation of these enzymes are regulated in a tightly coordinated manner during keratinocyte differentiation. We here show the system detecting the activity of the TGases using specific glutamine-donor substrate peptides in a three-dimensional culture system of keratinocytes. In this review, we summarize the roles of the epidermal enzymes and introduce a detection method that will provide a system for evaluating the skin barrier function. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Hirofumi Teshima, Manami Kato, Hideki Tatsukawa, Kiyotaka Hitomi. Analysis of the expression of transglutaminases in the reconstructed human epidermis using a three-dimensional cell culture. Analytical biochemistry. 2020 Aug 15;603:113606