LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control.

Nature cell biology 2020 Feb

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The Hippo and mammalian target of rapamycin complex 1 (mTORC1) pathways are the two predominant growth-control pathways that dictate proper organ development. We therefore explored potential crosstalk between these two functionally relevant pathways to coordinate their growth-control functions. We found that the LATS1 and LATS2 kinases, the core components of the Hippo pathway, phosphorylate S606 of Raptor, an essential component of mTORC1, to attenuate mTORC1 activation by impairing the interaction of Raptor with Rheb. The phosphomimetic Raptor-S606D knock-in mutant led to a reduction in cell size and proliferation. Compared with Raptor+/+ mice, RaptorD/D knock-in mice exhibited smaller livers and hearts, and a significant inhibition of elevation in mTORC1 signalling induced by Nf2 or Lats1 and Lats2 loss. Thus, our study reveals a direct link between the Hippo and mTORC1 pathways to fine-tune organ growth.

Citation

Wenjian Gan, Xiaoming Dai, Xiangpeng Dai, Jun Xie, Shasha Yin, Junjie Zhu, Chen Wang, Yuchen Liu, Jianping Guo, Min Wang, Jing Liu, Jia Hu, Ryan J Quinton, Neil J Ganem, Pengda Liu, John M Asara, Pier Paolo Pandolfi, Yingzi Yang, Zhigang He, Guangping Gao, Wenyi Wei. LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control. Nature cell biology. 2020 Feb;22(2):246-256