Bacterial expression and characterization of an anti-CD22 single-chain antibody fragment.

Diana Mikiewicz, Natalia Łukasiewicz, Marcin Zieliński, Violetta Cecuda-Adamczewska, Anna Bierczyńska-Krzysik, Agnieszka Romanik-Chruścielewska, Małgorzata Kęsik-Brodacka

Protein expression and purification 2020 Jun

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Single-chain variable fragment (scFv) antibodies are fusion proteins of the variable regions of the heavy and light chains of immunoglobulins connected with a short linker peptide. They possess unique and superior features compared to whole antibodies for immunotherapy of various carcinomas, including hematologic B-cell malignancies. In the presented study we obtained efficient production of the recombinant anti-CD22 scFv in Escherichia coli expression system. The active recombinant protein was successfully recovered from inclusion bodies. Assays were performed to assess the in vitro targeting properties and specificity of the obtained anti-CD22 scFv antibody in the CD22 positive and negative lymphoma cell lines. Copyright © 2020 Research Network Aukasiewicz Institute of Biotechnology and Antibiotics. Published by Elsevier Inc. All rights reserved.

Citation

Diana Mikiewicz, Natalia Łukasiewicz, Marcin Zieliński, Violetta Cecuda-Adamczewska, Anna Bierczyńska-Krzysik, Agnieszka Romanik-Chruścielewska, Małgorzata Kęsik-Brodacka. Bacterial expression and characterization of an anti-CD22 single-chain antibody fragment. Protein expression and purification. 2020 Jun;170:105594