Light-Up Lipid Droplets Dynamic Behaviors Using a Red-Emitting Fluorogenic Probe.

Xin Zhang, Lin Yuan, Jianxia Jiang, Jiwen Hu, Anna du Rietz, Hongzhi Cao, Ruilong Zhang, Xiaohe Tian, Fengling Zhang, Yuguang Ma, Zhongping Zhang, Kajsa Uvdal, Zhangjun Hu

Analytical chemistry 2020 Mar 03

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Intracellular lipid metabolism occurs in lipid droplets (LDs), which is critical to the survival of cells. Imaging LDs is an intuitive way to understand their physiology in live cells. However, this is limited by the availability of specific probes that can properly visualize LDs in vivo. Here, an LDs-specific red-emitting probe is proposed to address this need, which is not merely with an ultrahigh signal-to-noise (S/N) ratio and a large Stokes shift (up to 214 nm) but also with superior resistance to photobleaching. The probe has been successfully applied to real-time tracking of intracellular LDs behaviors, including fusion, migration, and lipophagy processes. We deem that the proposed probe here offers a new possibility for deeper understanding of LDs-associated behaviors, elucidation of their roles and mechanisms in cellular metabolism, and determination of the transition between adaptive lipid storage and lipotoxicity as well.

Citation

Xin Zhang, Lin Yuan, Jianxia Jiang, Jiwen Hu, Anna du Rietz, Hongzhi Cao, Ruilong Zhang, Xiaohe Tian, Fengling Zhang, Yuguang Ma, Zhongping Zhang, Kajsa Uvdal, Zhangjun Hu. Light-Up Lipid Droplets Dynamic Behaviors Using a Red-Emitting Fluorogenic Probe. Analytical chemistry. 2020 Mar 03;92(5):3613-3619