The potential neuroprotective role of mesenchymal stem cell-derived exosomes in cerebellar cortex lipopolysaccharide-induced neuroinflammation in rats: a histological and immunohistochemical study.

Abeer Moustfa El-Mahalaway, Nahla El-Eraky El-Azab

Ultrastructural pathology 2020 Mar 03

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Lipopolysaccharide (LPS) is an endotoxin that prompts neuroinflammation and initiates neurodegenerative disorders. Exosome is a recent therapeutic agent for many diseases such as neurological diseases. This study aimed to evaluate the potential protective role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-EXs) in cerebellar cortex LPS-induced neuroinflammation in rats. Twenty-seven adult male rats were divided into three groups: Group I: control rats; Group II: LPS-treated rats; Group III: LPS/BMSC-EXs-treated rats. Cerebellar specimens were taken and processed for histological and immunohistochemical analysis. Morphometrical studies and statistical analysis were done. Groups II showed neuronal degeneration and apoptosis. The mean number of Purkinje cells was significantly (P<0.01) decreased, while glial fibrillary acidic protein (GFAP) immunoexpression was significantly increased in the neuroglial cells. Ultrastructural examination showed shrunken Purkinje cells with irregular nuclei and disrupted mitochondria. Group III showed improvement of most of the changes mentioned previously. EXs therapy is a promising neuroprotective tool for treatment of LPS-induced neuroinflammation.

Citation

Abeer Moustfa El-Mahalaway, Nahla El-Eraky El-Azab. The potential neuroprotective role of mesenchymal stem cell-derived exosomes in cerebellar cortex lipopolysaccharide-induced neuroinflammation in rats: a histological and immunohistochemical study. Ultrastructural pathology. 2020 Mar 03;44(2):159-173