Activated Platelet-Derived Vesicles for Efficient Hemostatic Activity.

Joo Hang Lee, Heesun Jung, Jihyeon Song, Eun Seo Choi, Gayeon You, Hyejung Mok

Macromolecular bioscience 2020 Mar

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In this study, activated platelet-derived vesicles (Act-VEs) are developed as a novel hemostatic biomaterial. Spherical Act-VEs (114.40 ± 11.69 nm in size) with surface charges of -24.73 ± 1.32 mV are successfully prepared from thrombin-activated murine platelets with high surface expression of active glycoprotein IIb/IIIa (GP IIb/IIIa, also known as αIIbβ3) and P-selectin. Although nanosized vesicles from resting platelets (VEs) and Act-VEs showed similar sizes and surface charges, Act-VEs formed much larger aggregates in the presence of thrombin and CaCl2 , compared to VEs. After incubation with fibrinogen, Act-VEs formed much denser fibrin networks compared to platelets or VEs, probably due to active αIIbβ3 on the surfaces of the Act-VEs. After intravenous injection of the Act-VEs, tail bleeding time and the blood loss are greatly reduced by Act-VEs in vivo. In addition, Act-VEs showed approximately sevenfold lower release of pro-inflammatory interleukin-1β (IL-1β) during incubation for 4 days, compared to platelets. Taken together, the formulated Act-VEs can serve as a promising hemostatic biomaterial for the efficient formation of fibrin clots without releasing pro-inflammatory cytokine. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Joo Hang Lee, Heesun Jung, Jihyeon Song, Eun Seo Choi, Gayeon You, Hyejung Mok. Activated Platelet-Derived Vesicles for Efficient Hemostatic Activity. Macromolecular bioscience. 2020 Mar;20(3):e1900338