Alexander Beller, Andrey Kruglov, Pawel Durek, Victoria von Goetze, Katharina Werner, Gitta Anne Heinz, Justus Ninnemann, Katrin Lehmann, René Maier, Ute Hoffmann, René Riedel, Kevin Heiking, Jakob Zimmermann, Britta Siegmund, Mir-Farzin Mashreghi, Andreas Radbruch, Hyun-Dong Chang
European journal of immunology 2020 JunIn humans and mice, mucosal immune responses are dominated by IgA antibodies and the cytokine TGF-β, suppressing unwanted immune reactions but also targeting Ig class switching to IgA. It had been suggested that eosinophils promote the generation and maintenance of mucosal IgA-expressing plasma cells. Here, we demonstrate that not eosinophils, but specific bacteria determine mucosal IgA production. Co-housing of eosinophil-deficient mice with mice having high intestinal IgA levels, as well as the intentional microbiota transfer induces TGF-β expression in intestinal T follicular helper cells, thereby promoting IgA class switching in Peyer's patches, enhancing IgA+ plasma cell numbers in the small intestinal lamina propria and levels of mucosal IgA. We show that bacteria highly enriched for the genus Anaeroplasma are sufficient to induce these changes and enhance IgA levels when adoptively transferred. Thus, specific members of the intestinal microbiota and not the microbiota as such regulate gut homeostasis, by promoting the expression of immune-regulatory TGF-β and of mucosal IgA. © 2020 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Alexander Beller, Andrey Kruglov, Pawel Durek, Victoria von Goetze, Katharina Werner, Gitta Anne Heinz, Justus Ninnemann, Katrin Lehmann, René Maier, Ute Hoffmann, René Riedel, Kevin Heiking, Jakob Zimmermann, Britta Siegmund, Mir-Farzin Mashreghi, Andreas Radbruch, Hyun-Dong Chang. Specific microbiota enhances intestinal IgA levels by inducing TGF-β in T follicular helper cells of Peyer's patches in mice. European journal of immunology. 2020 Jun;50(6):783-794
PMID: 32065660
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