Correlation Engine 2.0
Clear Search sequence regions

  • acid (1)
  • amino acid (1)
  • formates (2)
  • formic acid (7)
  • mass (1)
  • peptides (5)
  • solvents (3)
  • Sizes of these terms reflect their relevance to your search.

    The ability of concentrated formic acid to formylate reactive amino acid residues is known from previous reports. In contrast, solvents containing a low concentration of formic acid are generally recognized as a safe environment for proteomic applications. The primary objective of this study was to explain the excessive formylation rate in tryptic peptides that did not come into contact with concentrated formic acid. We found out that the peptide formylation was associated with dissolving the peptides in a solvent containing mere 0.1% formic acid. Similar conclusions were drawn after analyzing publicly available proteomic data. We further demonstrated that these unwanted modifications can be averted via handling the samples at a low temperature or, obviously, via replacing formic acid in the solvent with trifluoroacetic acid. These simple countermeasures can contribute to a reduction in the part of the MS/MS spectra that remain unassigned to a peptide sequence.


    Juraj Lenčo, Maria A Khalikova, František Švec. Dissolving Peptides in 0.1% Formic Acid Brings Risk of Artificial Formylation. Journal of proteome research. 2020 Mar 06;19(3):993-999

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 32068400

    View Full Text