MiR-501-5p acts as an energetic regulator in head and neck squamous cell carcinoma cells growth and aggressiveness via reducing CLCA4.

Bo Li, Yuan-Pei Jiang, Jie Zhu, Lei Meng

Molecular biology reports 2020 Mar

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Recent research have proved that miR-501-5p acted as a potent tumor biomarker in several cancers, excluding head and neck squamous cell carcinoma (HNSCC). The study intends to discover the potential function and mechanism of miR-501-5p in HNSCC. Data from TCGA database and qRT-PCR estimated the expression of miR-501-5p and Calcium activated Chloride Channel A4 (CLCA4). Cell proliferation, clone formation and transwell assays were performed to explore HNSCC cells biological behaviors. Luciferase assay was carried out to identify the interaction between miR-501-5p and CLCA4. miR-501-5p was profoundly up-regulated in HNSCC samples and promoted cells proliferation and metastasis. CLCA4, as a target of miR-501-5p, was connected with worse outcomes in HNSCC patients. Co-transfection assay proved that miR-501-5p/CLCA4 functioned as crucial regulators to affect HNSCC cells biological behaviors. Our study illustrated that miR-501-5p exhibited a tumor-promoting role on HNSCC by targeting CLCA4, providing a new insight for revealing the pathogenesis and treatment of HNSCC.

Citation

Bo Li, Yuan-Pei Jiang, Jie Zhu, Lei Meng. MiR-501-5p acts as an energetic regulator in head and neck squamous cell carcinoma cells growth and aggressiveness via reducing CLCA4. Molecular biology reports. 2020 Mar;47(3):2181-2187