Molecular Simulation of Mechanical Properties and Membrane Activities of the ESCRT-III Complexes.

Taraknath Mandal, Wilson Lough, Saverio E Spagnolie, Anjon Audhya, Qiang Cui

Biophysical journal 2020 Mar 24

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The endosomal sorting complex required for transport (ESCRT) machinery carries out the membrane scission reactions that are required for many biological processes throughout cells. How ESCRTs bind and deform cellular membranes and ultimately produce vesicles has been a matter of active research in recent years. In this study, we use fully atomistic molecular dynamics simulations to scrutinize the structural details of a filament composed of Vps32 protomers, a major component of ESCRT-III complexes. The simulations show that both hydrophobic and electrostatic interactions between monomers help maintain the structural stability of the filament, which exhibits an intrinsic bend and twist. Our findings suggest that the accumulation of bending and twisting stresses as the filament elongates on the membrane surface likely contributes to the driving force for membrane invagination. The filament exposes a large cationic surface that senses the negatively charged lipids in the membrane, and the N-terminal amphipathic helix of the monomers not only acts as a membrane anchor but also generates significant positive membrane curvature. Taking all results together, we discuss a plausible mechanism for membrane invagination driven by ESCRT-III. Copyright © 2020 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Citation

Taraknath Mandal, Wilson Lough, Saverio E Spagnolie, Anjon Audhya, Qiang Cui. Molecular Simulation of Mechanical Properties and Membrane Activities of the ESCRT-III Complexes. Biophysical journal. 2020 Mar 24;118(6):1333-1343