Agrobacterium-mediated transformation of arthroconidia obtained from the edible mushroom Hypsizygus marmoreus.

Dapeng Bao, Zhiheng Huang, Yan Li, Chenli Zhou, Yan Li, Jianing Wan, Lihua Tang, Wenjun Mao, Ying Wang, Ming Gong, Gen Zou, Yoichi Honda, Ruiheng Yang, Junjun Shang

Journal of microbiological methods 2020 Apr

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Using the carboxin resistance gene from Pleurotus eryngii as a selective marker, we introduced foreign DNA into the arthroconidia of Hypsizygus marmoreus through Agrobacterium-mediated transformation. The function of the exogenous GUS (β-glucuronidase) gene driven by the CaMV35S promoter was detected in the transformants. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Dapeng Bao, Zhiheng Huang, Yan Li, Chenli Zhou, Yan Li, Jianing Wan, Lihua Tang, Wenjun Mao, Ying Wang, Ming Gong, Gen Zou, Yoichi Honda, Ruiheng Yang, Junjun Shang. Agrobacterium-mediated transformation of arthroconidia obtained from the edible mushroom Hypsizygus marmoreus. Journal of microbiological methods. 2020 Apr;171:105878