β-Actin facilitates etoposide-induced p53 nuclear import.

As a tumor suppressor, p53 preserves genomic integrity in eukaryotes. However, limited evidence is available for the p53 shuttling between the cytoplasm and nucleus. Previous studies have shown that β-actin polymerization negatively regulates p53 nuclear import through its interaction with p53. In this study, we found that DNA damage induces both β-actin and p53 accumulation in the nucleus. β-actin knockdown impaired the nuclear transport of p53. Additionally, β-actin could interact with p53 which was enhanced in response to genotoxic stress. Furthermore, N terminal deletion mutants of p53 shows reduced levels of association with β-actin. We further identified Ser15, Thr18 and Ser20 of p53 are critical to the β-actin: p53 interaction, which upon mutation into alanine abrogates the binding. Taken together, this study reveals that β-actin regulates the nuclear import of p53 through protein-protein interaction.

Citation

Wenjing Qi, Jinjiao Li, Xiaohua Pei, Yueshuang Ke, Qingpan Bu, Xiuzhen Ni. β-Actin facilitates etoposide-induced p53 nuclear import. Journal of biosciences. 2020;45

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PMID: 32098913

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