Redefining Single-Trial Memories in the Honeybee.

Maria Eugenia Villar, Paul Marchal, Haydee Viola, Martin Giurfa

Cell reports 2020 Feb 25

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Research on honeybee memory has led to a widely accepted model in which a single pairing of an odor stimulus with sucrose induces memories that are independent of protein synthesis but is unable to form protein-synthesis-dependent long-term memory (LTM). The latter is said to arise only after three or more pairings of odor and sucrose. Here, we show that this model underestimates the capacity of the bee brain to form LTMs after a unique appetitive experience. Using state-of-the art conditioning setups and individual-based analyses of conditioned responses, we found that protein-synthesis-dependent memories are formed already 4 h after the single conditioning trial and persist even 3 days later. These memories (4 h, 24 h, and 72 h) exhibit different dependencies on transcription and translation processes. Our results thus modify the traditional view of one-trial memories in an insect with a model status for memory research. Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Maria Eugenia Villar, Paul Marchal, Haydee Viola, Martin Giurfa. Redefining Single-Trial Memories in the Honeybee. Cell reports. 2020 Feb 25;30(8):2603-2613.e3