Dissection of the Human T-Cell Receptor γ Gene Repertoire in the Brain and Peripheral Blood Identifies Age- and Alzheimer's Disease-Associated Clonotype Profiles.

Maria Aliseychik, Anton Patrikeev, Fedor Gusev, Anastasia Grigorenko, Tatiana Andreeva, Arya Biragyn, Evgeny Rogaev

Frontiers in immunology 2020

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The immune system contributes to neurodegenerative pathologies. However, the roles of γδ T cells in Alzheimer's disease (AD) are poorly understood. Here, we evaluated somatic variability of T-cell receptor γ genes (TRGs) in patients with AD. We performed deep sequencing of the CDR3 region of TRGs in patients with AD and control patients without dementia. TRG clones were clearly detectable in peripheral blood (PB) and non-neuronal cell populations in human brains. TRG repertoire diversity was reduced during aging. Compared with the PB, the brain showed reduced TRGV9 clonotypes but was enriched in TRGV2/4/8 clonotypes. AD-associated TRG profiles were found in both the PB and brain. Moreover, some groups of clonotypes were more specific for the brain or blood in patients with AD compared to those in controls. Our pilot deep analysis of T-cell receptor diversities in AD revealed putative brain and AD-associated immunogenic markers. Copyright © 2020 Aliseychik, Patrikeev, Gusev, Grigorenko, Andreeva, Biragyn and Rogaev.

Citation

Maria Aliseychik, Anton Patrikeev, Fedor Gusev, Anastasia Grigorenko, Tatiana Andreeva, Arya Biragyn, Evgeny Rogaev. Dissection of the Human T-Cell Receptor γ Gene Repertoire in the Brain and Peripheral Blood Identifies Age- and Alzheimer's Disease-Associated Clonotype Profiles. Frontiers in immunology. 2020;11:12