Recovery of DNA from Low-Melting-Temperature Agarose Gels: Organic Extraction.

In this protocol, DNA fragments are separated according to size by electrophoresis through low-melting-temperature agarose, and then recovered by melting the agarose and extracting with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 to 5.0 kb. Yields of DNA fragments outside this range are usually lower, but often are sufficient for many purposes. © 2020 Cold Spring Harbor Laboratory Press.

Citation

Michael R Green, Joseph Sambrook. Recovery of DNA from Low-Melting-Temperature Agarose Gels: Organic Extraction. Cold Spring Harbor protocols. 2020 Mar 02;2020(3):100461

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PMID: 32123015

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