Expression of TRPC3 in cortical lesions from patients with focal cortical dysplasia.

Chao Liang, Xin Chen, Chun-Qing Zhang, Ning An, Shi-Yong Liu, Da-Hai Zheng, Hui Yang

Neuroscience letters 2020 Apr 17

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Focal cortical dysplasia (FCD) is one of the main causes of medically intractable epilepsy. Some studies have reported that transient receptor potential canonical channel 3 (TRPC3) may play an important role in the occurrence of seizures. In this study, we investigated the expression patterns of TRPC3 in different types of FCD. Forty-five FCD specimens and 12 control samples from autopsies were used in our study. Western blotting, immunohistochemistry, and immunofluorescence staining were employed to detect protein expression and distribution. The amount of TRPC3 protein was markedly elevated in the FCD group. The immunohistochemistry results revealed that TRPC3 staining was strong in the malformed cells and microcolumns. Most of the TRPC3-positive cells were colabeled with glutamatergic and GABAergic markers. The overexpression and altered cellular distribution of TRPC3 in the FCD samples suggest that TRPC3 may be related to epileptogenesis in FCD. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Chao Liang, Xin Chen, Chun-Qing Zhang, Ning An, Shi-Yong Liu, Da-Hai Zheng, Hui Yang. Expression of TRPC3 in cortical lesions from patients with focal cortical dysplasia. Neuroscience letters. 2020 Apr 17;724:134880