Environmental contaminants modulate transport activity of zebrafish organic anion transporters Oat1 and Oat3.

Organic anion transporters (OATs) are transmembrane proteins which belong to SLC22 subfamily. They are responsible for the uptake of various endo- and xenobiotics into the cells of different organs and tissues. Following our previous work on characterization of zebrafish Oat1 and Oat3, in this study we analyzed interaction of various classes of environmental contaminants with these membrane transporters using the transport activity assay with HEK293 Flp-In cell line stably overexpressing zebrafish Oat1 and Oat3, respectively. Based on the initial screening of a series of 36 environmental contaminants on their ability to interact with zebrafish Oat1 and Oat3, the most potent interactors were selected, their IC50 values calculated and type of interaction determined. Finally, to further confirm the type of interaction and initially evaluate their toxic potential, the cytotoxicity assays were performed. Broad ligand selectivity and similarity of zebrafish Oat1 and Oat3 with mammalian orthologs was confirmed and potent interactors among environmental contaminants identified. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Jelena Dragojević, Petra Marić, Jovica Lončar, Marta Popović, Ivan Mihaljević, Tvrtko Smital. Environmental contaminants modulate transport activity of zebrafish organic anion transporters Oat1 and Oat3. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 2020 May;231:108742

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PMID: 32165351

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